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대한생식의학회지 제27권 제1호 2010년
인간 난소의 과립 세포 배양 중 Leptin이 스테로이드 생성에 미치는 영향
연세대학교 의과대학 산부인과학교실, 아주대학교 의과대학 산부인과학교실1
김세광, 김명신1, 황경주1, 권혁찬1, 조동제,
Effect of Leptin on the Steroidogenesis of Cultured Human Granulosa Cells
Sei Kwang Kim, Myougn Shin Kim1, Kyung Joo Hwang1, Hyuck Chan Kwon1, Dong Jae Cho
Department of Obstetrics and Gynecology, Yonsei university College of Medicine, Seoul, Department of Obstetrics and Gynecology, Ajou University School of Medicine, Suwon, Korea1
Objective: To elucidate the location of leptin and leptin receptors of ovary specimens obtained from patients undergoing hysterectomy by immunohistochemical staining and to determine the effect of leptin on the steroidogenesis of cultured granulose cells. Method: In the culturing process of the granulose cells, FSH (1 IU/ml) and leptin (50 ng/ml), IGF-I (50 ng/ml) was administered to each study group (Group 1: FSH; Group II: FSH, leptin; Group III: FSH, IGF-I; Group IV: FSH, IGF-I, leptin), and the levels of estradiol, progesterone, androstenedione in the culture media was measured by radioimmunoassay. Statistical analysis was conducted by one-way ANOVA with Scheffe test. Results: The results showed that leptin and leptin receptors were both found to be strongly stained in granulose and theca cells, and also in some interstitial cells, and also in some interstitial cells. Lepin receptors were also observed in cultured granulose cells. While there was no statistically significant difference in the androstenedione concentrations between the groups, estradiol concentrations was significantly decreased in Group IV (2202.0¡¾151.14 pg/ml) compared to Group III (2859,0¡¾122.6 pg/ml), and progesterone concentrations were also significantly decreased in Group II (4696.3¡¾190.6 ng/ml) and Group IV (4517¡¾206.78 ng/ml) compared to Group III (5546.0¡¾179,5 ng/ml). Conclusion: The study result of this study suggest that leptin is directly involved in the regulation of ovarian functions, in particular steroidogenesis.
키워드 : Leptin, Leptin receptor, Granulosa cell, Steroidogenesis
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