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대한생식의학회지 제35권 제4호 2010년
유리화동결 전 인공수축과 보조부화술이 융해 후 생쥐 포배아의 발달에 미치는 영향
부산대학교 의과대학 산부인과학교실1, 부산대학교병원 불임클리닉2
조덕현1•고경래2•정지혜2•최종렬1•주종길1•이규섭1*,
Effects of the Artificial Shrinkage and Assisted Hatching Before Vitrification on the Development of the Vitrified Mouse Expanding Blastocysts
Deok-Hyeon Jo1, Gyoung-Rae Ko2, Ji-Hye Jung2, Jong-Ryeol Choi1, Jong-Kil Joo1, Kyu-Sup Lee1*
1Department of Obstetrics and Gynecology, School of Medicine, Pusan National University, Busan, Korea, 2Clinic of Infertility, Pusan National University Hospital, Busan, Korea
Objective: This study was conducted to investigate the effects of the artificial shrinkage and assisted hatching (PZD; patial zona dissetion) before vitrification on the development of vitrified mouse expanding blastocyst. Methods: Mouse 2-cell embryos were collected and cultured in G1.1 and G2.2 to expanding blastocyst. For artificial shrinkage (AS) the micro injection pipette was inserted into blastocoele cavity and blastocoele fluid was aspirated. For assisted hatching (AH) PZD method was used. Control group was -AS/-AH and treatment groups were -AS/+AH, +AS/-AH and +AS/+AH. After AS and AH mouse blastocysts were equillibrated in G10 and G10E20 for 3 mins, respectively, and vitrified in G25E25 after loading on capped pulled-straw. Vitrified mouse blastocysts were thawed and cultured for 24 hrs. The survival and hatching rate was compared among one control and three treatment groups. Results: The survival rates were 99%, 92% in +AS/+AH and +AS/-AH groups and 54%, 58% in -AS/-AH and -AS/+AH group, respectively. The survival rate was significantly higher in +AS group than in -AS group (p<0.01). Hatching rates were 34%, 96% in -AS/-AH and -AS/+AH groups and 41%, 100% in +AS/-AH and +AS/+AH, respectively. The hatching rates was higher in +AH group than in -AH group (p<0.01). After thawing recovery rates were 100%. Loading on capped pulled-straw, that is effective and useful method on vitrification. Conclusion: This study showed that artificial shrinkage of blastocoele cavity and assisted hatching (PZD) significantly improved the development of the vitrified mouse expanding blastocysts.
키워드 : Vitrification, Artificial shrinkage, Assisted hatching, Blastocoele cavity, Capped pulled-straw
교신저자 : kslee@pusan.ac.kr
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