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대한생식의학회지 제29권 제2호 2010년
Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF)의 첨가가 생쥐 수정란의 발생과 착상관련 유전자 발현에 미치는 영향
을지병원 의과학연구소1, 을지대학교 의과대학 생리학교실2, 산부인과학교실4, 서울보건대학 임상병리과3
김동훈, 고덕성1,이회창1,이호준2,강희규3,김태전3,박원일4,김세웅4
Effect of GM-CSF on the Embryonic Development and the Expression of Implantation Related Genes of Mouse Embryos
DH Kim
Eulji General Hospital, Physiology2 and OB/GYN4,Eulji University,Seoul Health College
Objective: The purpose of the current series of experiments were to assess the effect of GM-CSF, as a medium supplement, on the development of mouse embryos and the expression of LIF and IL-1b mRNA. Materials and Methods: Mouse 2-cell embryos were collected from the oviducts of 6 weeks old ICR mice at 48 hours after hCG injection. Embryos were cultured in P-1 medium supplemented with mouse GM-CSF (0, 1, 5, 10 ng/ml). The embryo development to blastocysts and hatching blastocysts was assessed and the cell number in blastocyst was also examined. Using RT-PCR, the expressions of LIF and IL-1b mRNA in blastocyst were evaluated in the GM-CSF supplemented group and control group. Results: In mouse, the addition of GM-CSF increased the percentage of blastocysts (65.5%, 68.6%, 73.0% and 76.1% for control and 1, 5 and 10 ng/ml, respectively), and increased the proportion of hatching blastocysts (35.2%, 36.4%, 43.2% and 53.0% for control and 1, 5 and 10 ng/ml, respectively). The mean cell numbers in blastocyst were significantly increased in GM-CSF supplemented groups compared to control group. LIF and IL-1b expression in blastocyst were significantly higher in GM- CSF supplemented group than in control group. Conclusion: The results of experiment by mouse embryos showed beneficial effects of GM-CSF as a medium supplement. Furthermore, the addition of GM-CSF significantly increased the expression of LIF and IL-1b in mouse embryos. These results suggest that GM-CSF might be a important molecule in embryo implantation.
키워드 : GM-CSF, Mouse embryo, LIF, IL-1b
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