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대한생식의학회지 제29권 제3호 2010년
인간난포에서 Apoptosis 관련 단백질 및 Apoptotic 세포의 면역학적 발현
연세대학교 의과대학 부속 영동 세브란스 병원 산부인과학교실,연세대학교 의과대학 병리학교실
박성록, 이병석,양우익,김종화,박병주,박기현,조동제,송찬호
The Immunologic Expression of Apoptosis Related Proteins and Apoptotic Cells in Human Ovarian Follicles
Park SR
Obstetrics and Gynecology, Department of Pathology Yonsei University College of Medicine, Seoul
Objective: To investigate the expression of apoptosis related proteins and apoptotic cells on the human ovarian follicles. Materials and Methods: Thirty five Formalin-fixed paraffin-embedded human ovarian tissue blocks were selected from the surgical pathology files of the department of pathology, College of Medicine, Yonsei University, for the period from 1996 to 1998. All specimen were from premenopausal women aged from 32~45. Ovarian tissues were collected from the patients performing hysterectomy for benign uterine diseases. Immunohistochemical staining was performed for the detection of DNA fragmented cell, Bcl-2, Bax, Fas and Fas-ligand. Results: Bcl-2 and bax were not expressed on the surrounding cells and oocyte of the primary, primordial and preantral follicles. Fas and Fas-ligand (Fas-L) were not expressed on the surrounding cells on the primordial and primary follicles. But expressed on the surrounding granulosa cells and oocyte in the primordial and primary follicles. In the healthy follicles, Bcl-2 was expressed on the granulosa cells, however, Bax was not expressed. DNA fragmented cells were expressed on the inner granulosa cell layer of atretic follicles. Conclusion: Fas, Fas-ligand, and Bax may be responsible for the follicular atresia and Bcl-2 may be involved in the follicular survival in the human ovary.
키워드 : Apoptosis, Bcl-2, Bax, Fas, Fas-ligand, Human ovary
교신저자 : dr222@yumc.yonsei.ac.kr
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