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대한생식의학회지 제29권 제4호 2010년
마우스에서 배반포 형성, 세포 수 및 ICM의 비율에 미치는 배양액의 효과
대구대학교 생명자원학부,경북대학교병원 산부인과
박기상, 이택후,전상식,송해범
Effects of Medium on Blastocyst Formation, Cell Number and Percentage of ICM in Mice
Park KS
Division of Life Resources Daegu University Gyungbuk,Department of OB/Gyn, Kyungpook National Univ
이 논문의 요지는 2002년 9월 28~29일 서울 매리어트 호텔에서 개최된 제 88차 대한산부인과학회 학술대회에서 구두 발표되었음.
Objective: The aim of this study was to evaluate the influence of different media on blastulation, mean cell number, percentage of inner cell mass (ICM) of total cells and ICM : trophectoderm (TE) ratio in mice. Materials and methods: A total 552 two cell embryos were retrieved from ICR female mice (4 weeks old) at 48 hr after hCG injection (mated just after hCG injection) and cultured in MEM (n=276) or TCM (n=276) supplemented with 20% hFF. The grading of blastocysts from zona-intact (ZiB) to -escape (hatching and hatched, ZeB) was performed at 72 hours after culture. Total, TE and ICM cell numbers were analyzed by differential staining of blastocyst. Statistical analysis was performed using t-test with SigmaPlot-2001. P-values < 0.05 were accepted as statistically significant. Results: The blastulation rate in MEM (64.9¡¾4.95%) was significantly higher (p=0.0031) than that in TCM (57.2¡¾5.22%). No differences were found in the number of ZiB and ZeB between MEM (31.9¡¾2.62, 33.0¡¾4.58%), and TCM (27.2¡¾4.28, 30.1¡¾4.58%). A total 314 blastocysts (MEM=166; TCM= 148) were stained differentially. Mean cell number of blastocysts was significantly higher (p=0.0002) in TCM (73.1¡¾3.3) than in MEM (61.7¡¾2.5). Differential staining was successfully performed in 155 blastocysts (MEM=77; TCM=78). The percentage of ICM was significantly higher in MEM than in TCM (20.9¡¾1.3 vs. 17.1¡¾1.2%, p=0.0281). The ICM : TE ratio was higher in TCM than in MEM (1 : 4.85¡¾0.68 vs. 1 : 3.78¡¾0.78, NS). Conclusion: These results show that MEM increase the blastocyst formation and percentage of ICM of total cells comparing with TCM in mice.
키워드 : Mice, Medium, Blastulation, Inner cell mass (ICM), Trophectoderm (TE)
교신저자 : keespark@yahoo.com
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