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대한생식의학회지 제30권 제2호 2010년
동결보존된 생쥐 고환조직 세포의 광학 및 전자현미경적 관찰
삼성제일병원 생식생물학 및 불임연구실, 산부인과, 순천향대학교 생명과학과
한상철, 송상진,이선희,오승한,궁미경,박용석
Light and Electron Microscopic Observation in the Frozen-thawed Mouse Testicular Tissues
Sang Chul Han
Laboratory of Reproductive Biology and Infertility, Department of Obstetrics & Gynecology Samsung Cheil Hospital & Women's Healthcare Center, Seoul, Department of Life Science, Soonchunhyang Univ
Objective: The aim of this study was to investigate the morphological aspects of testicular tissue before and after freezing-thawing by light and transmission electron microscopy. Methods: Tissue biopsies were carried out on mouse testis for freezing. Samples in medium containing 20% glycerol were frozen by computer-controlled freezing program. The effect of freezing- thawing on the structural change of testicular tissues were examined by light and electron microscopy. Results: The freezing-thawing procedure had no significant effect on tubular diameter. However, it caused folding of the lamina propria, and notable damage to Sertoli cells, spermatogonia and spermatocytes. The cells were detached, desquamated from the basal lamina and had increased vacuolization. Round spermatids, elongated spermatids and spermatozoa were less affected, and most of them maintained their normal structure. Conclusions: The structure of spermatogonia, spermatocyte and basal compartments in seminiferous epithelium was significantly altered by freezing-thawing procedure of mouse testicular tissues. Thus, we need to develop a more reliable method for the cryopreservation of testicular tissues.
키워드 : Freezing-thawing, Ultrastructure, Testicular tissues, Seminiferous tubule
교신저자 : sch-han@hanmail.net
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