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대한생식의학회지 제27권 제3호 2010년
Extracellular Matrix 배지에서 생쥐 배아의 발생 및 아폽토시스
1울산대학교 외과대학 산부인과, 2경기대학교 자연과학부 생물학과, 3바이오메드 연구소
강병문1, 손인표2, 정병목2, 최규완3, 계명찬2,
Preimplantation Development and Apoptosis of Mouse Embryos in the Medium Containing Extracellular Matrix
Byung Moon Kang1, In Pyo Sohn2, Byung Mok Chung2, Kyoo Wan Choi3, Myung Chan Gye2
1Department of Obsterics and Gynecology, College of Medicine, Ulsan University, 2Department of Biology, Natural Sciences, Kyonggi University, 3Biomed Institute
Objective: To verify the effect of Matrigel, a ECM complex from Engelbreth-Holm-Swarm (EHS) mouse sarcoma on the preimplantation development and apoptosis of mouse fertilized eggs. Method: Late pronucleus stage eggs were cultured through the blastocyst stage in the presence of Matrigel (0.5%, v/v). Characteristics of apoptosis and cell number assesed by Hoecst staining and TUNEL labeling at the blastocyst stage, respectively. Results: Morphological development, number of cells per embryo was significantly increased but rate and number of TUNEL positive nuclei of the embryo were decreased in the presence of Matrigel. Conclusion: This result suggested that at low concentration of Matrigel improves both viability and morphological development in the preimplantation mouse embryos.
키워드 : Matrigel, Mouse embryos, Development, Apoptosis
교신저자 : mcgye@kuic.kyonggi.ac.kr
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