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대한생식의학회지 제17권 제1호 2010년
생쥐 상실배의 초급속동결
경희대학교 의과대학 산부인과학교실 불임크릭??;경희대학교 의과대학 산부인과학교실 불임크릭??;경희대학교 의과대학 산부인과학교실 불임크릭??;한국과학기술연구원 유전공학센타 발생공학연구실;
백청순;서병희;이재현;이경광;,
Ultrarapid Freezing of Mouse Morulae
Baik, C.S.;Suh, M.D.;Lee, J.H.;Lee, K.K.;
Infertility Clinic, Department of Obstetrics & Gynecology, College of Medicine Kyung Hee University;Infertility Clinic, Department of Obstetrics & Gynecology, College of Medicine Kyung Hee University;Infertility Clinic, Department of Obstetrics & Gynecology, College of Medicine Kyung Hee University;Developmental Biotechnology Lab., Genetic Engineering Center, KIST;
We cryopreserved mouse morulae by a simple ultra-rapid method of freezing embryos directly in $LN_2$ after holding 2min in a $LN_2$ vapor, and thawed them in $37^{\circ}C$ water bath. The time requirements for permeation and dehydration by 2.0 M glycerol and 0.2 M sucrose before freezing were studied. When the embryos were equilibrated for 10 min, the optimun post-thaw survival was obtained. Embryos those developed normally to blastocyst after in vitro culture for over 24hrs were regarded as survival ones. Two experiments to assess post-thaw survival following predehydration in various mixtures of glycerol and sucrose were also accomplished. When sucrose was held constant (0.2 M) and glycerol concentration varied (1.5-3.5 M), post-thaw survival was best (78.0%) in 3.0 M glycerol. When glycerol was held constant (3.0M) and sucrose concentration varied (0.0-1.0M), optimun post-thaw survival (78.0%) was found in 0.2 M sucrose.
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