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대한생식의학회지 제17권 제1호 2010년
혈청내의 Progesterone 측정을 위한 Chemiluminescence Immunoassay의 개발에 관한 연구
경희대학교 의과대학 산부인과학교실(불임크리??);경희대학교 의과대학 산부인과학교실(불임크리??);경희대학교 의과대학 산부인과학교실(불임크리??);건국대학교 축산대학 축산가공학과;
이기순;서병희;이재현;김종배;,
Development of Chemiluminescence Immunoassay for Progesterone in Serum
Lee, K.S.;Suh, B.H.;Lee, J.H.;Kim, J.B.;
Infertility Clinic, Department f Obstetrics and Gynecology, Collage of Medicine, Kyung Hee University;Infertility Clinic, Department f Obstetrics and Gynecology, Collage of Medicine, Kyung Hee University;Infertility Clinic, Department f Obstetrics and Gynecology, Collage of Medicine, Kyung Hee University;Department of Animal Products Science, Science, Collage of Animal Husbandry, Kun-Kuk University;
Development of a solid-phase chemiluminescence immunoassay for the detection of progesterone in serum extract was described. The chemiluminescence immunoassay was establised utilizing anti-progesterone monoclonal antibody that coated on polystyrene tubes and progesterone-ABEI conjugate as tracer. The light yield generated from antibody bound conjugate was counted on clinilumat luminometer by oxidation with microperoxidase and peroxide. The chemiluminescence immunoassay was high specific and accurate and detects as little as 3.9ng/ml of progesterone. The intra-assay CV ranged from 6% to 11.5% and inter-assay CV ranged from 13.6% to 18.7%. This assay system was good correlated with conventional kit radioimmunoassay system (r=0.98).
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