| 학회지 검색 | HOME > 논문 및 학회지 > 학회지 검색 |
대한생식의학회지 제19권 제1호 2010년
TEST-Yolk Buffer에 의한 인간 정자의 수정능 증진효과에 관한 연구
서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;
방명걸;김기철;신창재;문신용;이진용;장윤석;,
Improved Fertilization Rate in Human In vitro Fertilization with the Use of a TEST-Yolk Buffer
Pang, Myung-Geol;Kim, Ki-Chul;Shin, Chang-Jae;Moon, Shin-Yong;Lee, Jin-Yong;Chang, Yoon-Seok;
Department of Obstetrics & Gynecology, College of Medicine, Seoul National University;Department of Obstetrics & Gynecology, College of Medicine, Seoul National University;Department of Obstetrics & Gynecology, College of Medicine, Seoul National University;Department of Obstetrics & Gynecology, College of Medicine, Seoul National University;Department of Obstetrics & Gynecology, College of Medicine, Seoul National University;Department of Obstetrics & Gynecology, College of Medicine, Seoul National University;
The present study was undertaken to clarify the role of TEST-Yolk Buffer(TYB) as a factor for the improvement of human sperm fertility potential. We examined the effects of low temperature capacitation using TYB on sperm motility (%), motility pattern, normal morphology, true acrosome reaction, sperm penetration assay and human in vitro fertilization. Comparing the TYB method and swim-up method, the sperm motility(%) of selected sperm was not significantly different, but statistically significant differences were found in curvilinear velocity, linearity, lateral head displacement, normal morphology(%) and true acrosome reaction(%)(p<0.05). Results obtained from the sperm penetration assay demonstrated that the penetration index and penetration rate were increased significantly(p<0.05) when the spermatozoa were incubated in TYB, as compared with swim-up method. And fertilization of intact human oocytes was more succesful when spermatozoa were pretreated with TYB at
키워드 :
교신저자 :
전문 파일 : 
