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대한생식의학회지 제21권 제1호 2010년
투명대하 미세수정(SUZI)시 정자의 상태 및 처리방법에 따른 수정률과 임신률
PL 산부인과 체외수정연구실;PL 산부인과 체외수정연구실;PL 산부인과 체외수정연구실;PL 산부인과 체외수정연구실;PL 산부인과 체외수정연구실;PL 산부인과 체외수정연구실;한양대학교 자연과학대학 생물학과;
최규완;김수경;양현원;차영범;이승재;박종민;김문규;,
Influence of Sperm Parameters and Capacitation Methods on the Outcome of Subzonal Insemination(SUZI)
Choi, Kyoo-Wan;Kim, Soo-Kyung;Yang, Hyun-Won;Cha, Young-Beom;Lee, Seung-Jae;Park, Jong-Min;Kim, Moon-Kyoo;
IVF Research Laboratory, Park & Lee Infertility Clinic;IVF Research Laboratory, Park & Lee Infertility Clinic;IVF Research Laboratory, Park & Lee Infertility Clinic;IVF Research Laboratory, Park & Lee Infertility Clinic;IVF Research Laboratory, Park & Lee Infertility Clinic;IVF Research Laboratory, Park & Lee Infertility Clinic;Department OF Biology, College OF Natural Sciences, Han-Yang University;
Subzonal insemination(SUZI) has been proposed for patients with severe male factor and previous fertilization failure. However, very low fertilization rates still persisted. The aims of this study were firstly, to examine the relationships between the fertilization rate and sperm parmeters, sperm incubation media and time, secondly, to evaluate the outcome of 119 cycles of SUZI applied the modified sperm preparation method. The fertilization rates were influenced more sensitively by sperm preincubation media and time than by sperm parameters. According to preincubation media and time, the fertilization rates were 43.3% in 50% follicular fluid (HFF), 36.6% in 10% fetal cord serum(FCS), and with the time, increased in FCS, but decreased in HFF. In regrd with sperm parameters, the fertilization rates were 42.9% in normal and 37.6% in subnormal group. The best results were obtained from SUZI by the spermatozoa incubated in 50% HFF for 6-8 hours. So we tried 119 cycles of SUZI(normal; 39 cycles, subnormal; 80 cycles) using the preparation method of 6-8 hour incubation in 50% HFF. There were no signigicant differences in the fertilization rates between normal(125/269, 46.4%) and subnormal sperm(264/635, 41.6%). Contrary to the fertilization rates, pregnancy outcomes were different between both groups. Better results obtained from the subnormal group than the normal in the number of transferred embryos, that of good embryos, and developmental rate of the fertilized eggs. The pregnancy rates per transfer were totally 13.3%(13/98),20.0%(13/65) in subnormal group. In the normal group, 2 patients showed
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