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대한생식의학회지 제21권 제1호 2010년
ICR계 생쥐 1세포배를 이용한 수질의 평가
함춘여성 크리닉;함춘여성 크리닉;함춘여성 크리닉;함춘여성 크리닉;함춘여성 크리닉;함춘여성 크리닉;
김충현;정경순;박소현;황도영;김기철;민응기;,
Evaluation of Water Quality using ICR Mouse 1-cell Embryo
Kim, Chung-Hyon;Cheong, Kyung-Soon;Park, So-Hyun;Hwang, Do-Yeong;Kim, Ki-Chul;Min, Eung-Gi;
Hamchoon Women's Clinic;Hamchoon Women's Clinic;Hamchoon Women's Clinic;Hamchoon Women's Clinic;Hamchoon Women's Clinic;Hamchoon Women's Clinic;
To confirm the overcome of in vitro 2-cell block, ICR mouse I-cell embryos were cultured in CZB media. All embryos in CZB were overcome in vitro 2-cell block and 92% of embryos were developed to the blastocyst at day 4. However, in m-KRB group(control) only 20% of embryos were developed over 2-cell. Any embryos in m-KRB did not develop to the morular stage. Developments and degenerations of ICR mouse I-cell embryos were compared in CZB medium prepared with water of three quality:(l) Milli-Q ultrafiltration water(UF);(2) Milli-Q reverse osmosis water(RO);(3) tap water(TAP). The objective was to evaluate the potential of quality control using ICR mouse 1-cell embryos. The more water was purified, the better embryo developments were supported and the less embryos were degenerated. As a quality control system, the culture of ICR 1-cell mouse embryos in CZB was useful.
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