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대한생식의학회지 제21권 제1호 2010년

생쥐 난자 배양시 외분비 관련 요소들이 자발적 투명대 경화 현상에 미치는 영향

성신여자대학교 자연과학대학 생물학과;성신여자대학교 자연과학대학 생물학과;서울여자대학교 자연과학대학 생물학과;

강혜나;배인하;김해권;,

Effect of Exocytosis Factor on Spontaneous Zona Pellucida Hardening during in Vitro Culture of the Mouse Oocytes

Kang, Hye-Na;Bae, In-Ha;Kim, Hae-Kwon;

Department of Biology, College of Natural Sciences, Sungshin Women's University;Department of Biology, College of Natural Sciences, Sungshin Women's University;Department of Biology, College of Natural Sciences, Seoul Woman's University;

"Spontaneous" hardening of the zona pellucida of mouse oocytes during in vitro culture is most likely due to cortical granules exocytosis. Thus the purpose of the present study was to determine whether the exocytosis factor is involved in spontaneous zona pellucida hardening during in vitro culture of the mouse. The results obtained form these experiments were summarized as follows; 1. When a protein synthesis inhibitor(100${\mu}g$/ml puromycin) was added to the culture medium, it did not prevent spontaneous ZPH of mouse oocyte during in vitro culture. 2. Calmodulin antagonists (trifluoperazine and chlorpromazine) and calcium channel blocker (verapamil) had no inhibitory effect in spontaneous ZPH. 3. A microtubule assembly inhibitor, colcemid had some inhibitory effect on spontaneous ZPH. 4. Treatment with a microfillament formation blocker(cytochalasin-B) at 1${\mu}g$/ml concentration, resulted in the excellent inhibitory effect on spontaneous ZPH. However cytochalasin-B did not inhibit ethanol-induced ZPH.

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