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대한생식의학회지 제22권 제2호 2010년

사람의 정확한 염색체 분석을 위한 고해상 G-분염 염색체와 R-분염 염색체

제일병원 유전학연구실;제일병원 유전학연구실;건국대학교 생물학과;

박소연;최수경;이형환;,

High Resolution G- and R-banding for Accurate analysis in Human Chromosomes

Park, So-Yun;Choi, Soo-Kyung;Lee, Hyung-Hoan;

Genentic Research Laboratory, Cheil General Hospital;Genentic Research Laboratory, Cheil General Hospital;Department of biology, Kon-Kuk University;

A technique is described for producing high resolution G- and R-banded chromosomes in human peripheral lymphocyte cultures. Cultured lymphocyte cells were exposed to ethidium bromide ($10{\mu}g/ml$) and colcemid ($0.02{\mu}g/ml$) each for 2.5h and 0.5h prior to harvest for high resolution G-banded chromosomes. High resolution R-band patterns were obtained by BrdU substitution which was revealed by the fluorochrome-photolysis-Giemsa staining technique. These methods are easy to perform and highly reliable. The data on relative length of chromosomes at the four mitotic stages are presented in units of percentage of haploid autosome length. The characteristic patterns of GTG-bands (G-bands after trypsin and Giemsa) and RBG bands (R-bands after BrdU and Giemsa) were analyzed.

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