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대한생식의학회지 제22권 제2호 2010년
Epidermal Growth Factor(EGF)가 생쥐 초기배아의 발생에 미치는 영향
한양대학교 자연과학대학 생물학과;제일병원 불임연구실;이화여자대학교 의과대학 의학과;서울여자대학교 자연과학대학 생물학과;한양대학교 자연과학대학 생물학과;
변혜경;이호준;김성례;김해권;김문규;,
Effect of Epidermal Growth Factor(EGF) on Early Embryonic Development in Mouse
Byun, Hye-Kyung;Lee, Ho-Joon;Kim, Sung-Rye;Kim, Hae-Kwon;Kim, Moon-Kyoo;
Department of Biology, Hanyang University;Infertility Resaerch Laboratory, Cheil General Hospital;Department of Medicine, Ewha Woman's University;Department of Biology, Seoul Women's University;Department of Biology, Hanyang University;
Growth factors (GFs) produced by the embryo or by the maternal reproductive tract have been reported to regulate the embryonic development and differentiation. Among GFs, EGF as a mitogen plays a role in mitosis and functional differentiation of trophectoderm cells in mouse. The present study was carried out to investigate the effect of EGF on development of mouse embryos and to localize EGF in the mouse oocytes and embryos, which has been reported to be detected in the reproductive tract in mammals. To investigate the effect of EGF on the development of the embryo, mouse 2-cell embryos were cultured to blastocysts stage in Ham's F10 medium, treated with EGF(10-50 ng/ml) for 72 hrs. Immunocytochemistry was performed from oocyte to blastocyst stage with anti-EGF and anti-Mouse IgG, in order to determine the stage which EGF would be expressed in mouse. Exogenous EGF (more than 10 ng/ml) in the culture medium improved the developmental and hatching rates in the mouse embryos. As a result of immunocytochemistry, the embryonic EGF was expressed after the late 4-cell stage. EGF is thought to enhance preimplantation embryonic development and hatching. Exogenous EGF in the culture medium is thought to activate EGF receptor in the late 4-cell embryos and to enhance blastulation and hatching in mouse embryos. It is concluded that EGF enhances the developmental and hatching rates in the mouse embryos.
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