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대한생식의학회지 제23권 제3호 2010년

Electric Stimulation(음이온 pad)이 생쥐난자의 성숙 및 수정난의 난할에 미치는 영향

성신여자대학교 자연과학대학 생물학과;성신여자대학교 자연과학대학 생물학과;성신여자대학교 자연과학대학 생물학과;한양대학교 자연과학대학 생물학과;

배인하;박원;최성미;김문규;,

The Effect of Electric Stimulation(anion pad) on the Maturation of Follicular Oocytes and the Cleavage of Fertilized Embryos of the Mouse

Bae, In-Ha;Park, Won;Choi, Sung-Mi;Kim, Moon-Kyoo;

Department of Biology, College of Natural Sciences, Sungshin Women's University;Department of Biology, College of Natural Sciences, Sungshin Women's University;Department of Biology, College of Natural Sciences, Sungshin Women's University;Department of Biology, College of Natural Sciences, Hanyang University;

In the present study, mouse follicular oocytes and 2-cell embryos(late -zygote stage embryos included) were cultured on the electric pad for electric stimulation in the culture incubator. In addition, follicular oocytes and embryos were tested for maturation and development under higher temperature condition($39^{\circ}C$).Mouse follicular oocyte maturation were not affected by anion electric stimulation and there is no significant difference in GBVD and MI between the control and experiment group after 4hr culture. In the embryo culture, it was found that more morula and blastocyst were found in the electric stimulation group rather than the control(96hr). This may seem to be caused with cytoplasmic $Ca^{2+}$ transient rise by electric stimulation(anion pad). On the other hand higher temperature incubation ($39^{\circ}C$) on the anion pad caused all the embryos degenerated within $12h{\sim}24hr$ culture. This was quite different from large animal embryos(bovine, pig, sheep), in which beneficial effect of high temperature incubation for oocyte maturation and embryo development were found.

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