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대한생식의학회지 제24권 제1호 2010년
저정낭액이 생쥐 부정소 정자의 첨체반응에 미치는 영향
경기대학교 이과대학 생물학과;이화여자대학교 의과대학 의학과;한양대학교 자연과학대학 생물학과;
계명찬;김성례;김문규;,
Effect of Seminal Vesicle Fluid Components on Acrosome Reaction of Mouse Epididymal Sperm
Gye, Myung-Chan;Kim, Sung-Rye;Kim, Moon-Kyoo;
Department of Biology, College of Natural Sciences, Kyonggi University;Department of Medicine, College of Medicine, Ewha Women's University;Department of Biology, College of Natural Sciences, Hanyang University;
This study aimed to evaluate the effect of seminal vesicle fluid (SVF) on the acrosome reaction (AR) occurred spontaneously or induced by $Ca^{2+}$ ionophore A23187, follicular fluid, and progesterone in mouse epididymal sperm. SVF was divided into high (MW>10 kD) and low (MW<10 kD) fractions by ultrafiltration. The low MW fraction of SVF decreased the rate of spontaneous AR, however the high MW fraction did not. It suggested that the low MW fraction of SVF might have contained decapacitation factor(s) responsible for prolonging of time need for capacitation. When sperm preincubated for 60 min in the presence of SVF, the rate of AR induced by A23187 was decreased, but prolongation of preincubation time for 120 min significantly potentiated the AR by A23187. It suggested that addition of SVF into sperm preincubation medium imposed the epididymal sperm a condition similar to ejaculation. AR induced by human follicular fluid or progesterone was also inhibited by SVF. It suggested that substance in SVF might have affected AR of mouse sperm by inhibiting the interaction between AR inducing ligands and sperm surface receptors involved in acrosomal exocytosis.
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