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대한생식의학회지 제24권 제1호 2010년

Indomethacin이 생쥐 착상전 배아의 발생 및 부화에 미치는 영향

한양대학교 자연과학대학 생물학과;경기대학교 이과대학 생물학과;울산대학교 의과대학 산부인과학교실;한양대학교 자연과학대학 생물학과;

전용필;계명찬;김정훈;김문규;,

Effects of Indomethacin on Development and Hatching of Mouse Embryo

Cheon, Yong-Pil;Gye, Myung-Chan;Kim, Chung-Hoon;Kim, Moon-Kyoo;

Department of Biology, College of Natural Sciences, Hanyang University;Department of Biology, College of Natural Sciences, Kyonggi University;Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center;Department of Biology, College of Natural Sciences, Hanyang University;

The present study was designed to define the role of prostaglandin in the development and hatching of mouse embryo. The effects of indomethacin, an inhibitor of prostaglandin synthesis, on the development and hatching of morula and blastocyst were examined. In early morula stage, embryos were degenerated significantly at 100 ${\mu}M$ and 200 ${\mu}M$ indomethacin. However, the viability of embryos was not influenced by concentration in any other embryonic stages. In all embryonic stages, the hatching was suppressed with concentration dependent manner, but expansion was not suppressed. Particularly, in 84h embryos post hCG injection, the hatching was suppressed significantly compared with post hCG 72h or 96h embryos. When embryos were treated with 100 ${\mu}M$ indomethacin for a specific time (12h) in according to the development stage, the hatching was suppressed all groups. These suppressional effect was decreased as embryonic development stage was progressed. However, the expansion was not affected in all treatment group. This study suggests that hatching-related metabolic substances are synthesized from morula stage and intraembryonic signaling mediated prostaglandin was important for development and hatching of mouse embryo.

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