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대한생식의학회지 제24권 제2호 2010년
ICSI 프로그램에서 생쥐 투명대를 이용한 고환조직내 정자의 동결
가야자모병원 불임 유전연구소;가야자모병원 불임 유전연구소;가야자모병원 불임 유전연구소;가야자모병원 불임 유전연구소;카톨릭의과대학교 의과학연구원;진주산업대학교 낙농자원학과;가야자모병원 불임 유전연구소;가야자모병원 불임 유전연구소;
서태광;전병균;류은경;이은숙;류재웅;손시환;문진수;김광철;,
Cryopreservation of Testicular Spermatozoa using Mouse Zona Pellucida in Intracytoplasmic Sperm Injection Program
Suh, Tae-Kwang;Jeon, Byeong-Gyun;Ryu, Eun-Kyung;Lee, Eun-Sook;Ryoo, Zae-Yoong;Sohn, Sea-Hwan;Moon, Jin-Soo;Kim, Kwang-Chull;
Institute of Infertility and Cytogenetics, Kaya Mother-Child's Hospital;Institute of Infertility and Cytogenetics, Kaya Mother-Child's Hospital;Institute of Infertility and Cytogenetics, Kaya Mother-Child's Hospital;Institute of Infertility and Cytogenetics, Kaya Mother-Child's Hospital;Catholic Research Institute of Medical Science, College of Medicine, Catholic University;Department of Dairy Science & Technology, Chinju National University;Institute of Infertility and Cytogenetics, Kaya Mother-Child's Hospital;Institute of Infertility and Cytogenetics, Kaya Mother-Child's Hospital;
The survival rate and motility recovered after cryopreservation of testicular spermatozoa in testicular sperm extraction (TESE)-ICSI program is low. The purpose of this study was to assess the availability and efficiency of mouse empty zona pellucida in cryopreserving human TESE spermatozoa. Mouse empty zonae pellucidae were obtained by extraction of cytoplasm with or without cytochalasin B treatment. Motile sperm from proven-fertile donor and two azoospermic patients after TESE were individually inserted into empty zona pellucida and cryopreserved. Two to five days after cyropreservation, the frozen sperm were thawed and the rates of recovery and motility were observed. The ooplasmic extraction rates of control (N=80) and cytochalasin B treated oocytes (N=80) were 94.0% and 96.2%, respectively (p>0.05). The post-thaw recovery rates of spermatozoa and rates of motility recovery of ejaculate (N=70) and testicular (N=70) sperm were 97.1%, 97.1% and 95.7%, 94.3%, respectively (p>0.05). The results of this study showed that the mouse zone pellucida is useful for cryostorage of single testicular spermatozoa.
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