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대한생식의학회지 제24권 제2호 2010년
Vero Cell과의 공동배양이 체외에서 생쥐 배아발생에 미치는 영향
인제대학교 의과대학 산부인과학교실 서울 백병원;인제대학교 의과대학 산부인과학교실 서울 백병원;인제대학교 의과대학 산부인과학교실 서울 백병원;인제대학교 의과대학 산부인과학교실 서울 백병원;인제대학교 의과대학 산부인과학교실 서울 백병원;인제대학교 의과대학 산부인과학교실 서울 백병원;
이윤;박준홍;강혜나;김용봉;이응수;박성관;,
The Effects of Vero Cell Co-culture on Mouse Embryo Development
Lee, Yoon;Park, June-Hong;Kang, He-Na;Kim, Yong-Bong;Lee, Eung-Soo;Park, Sung-Kwan;
Department of Obstetrics and Gynecology, College of Medicine, InJe University, Seoul Paik Hospital;Department of Obstetrics and Gynecology, College of Medicine, InJe University, Seoul Paik Hospital;Department of Obstetrics and Gynecology, College of Medicine, InJe University, Seoul Paik Hospital;Department of Obstetrics and Gynecology, College of Medicine, InJe University, Seoul Paik Hospital;Department of Obstetrics and Gynecology, College of Medicine, InJe University, Seoul Paik Hospital;Department of Obstetrics and Gynecology, College of Medicine, InJe University, Seoul Paik Hospital;
Embryos of most mammalian species grown in vitro would undergo developmental arrest at the approximate time of genomic activation. Stage-specific cell block and the resulting rapid loss of embryo viability in conventional culture media have limited the duration for which embryos may be cultured prior to transfer. As a result, embryos are usually transferred to the uterus at the 4-to 8-cell stage to avoid the loss of viability associated with long-term in vitro culture. Early transfer has led to asynchrony of the endometrium-trophectoderm interaction at the time of implantation and a resultant reduction in the rate of implantation. To overcome these problems, a variety of co-culture systems has been devised in which embryos can develop for a longer period prior to embryo transfer. Vero cells, derived from African green monkey kidney, share a common embryologic origin with cells from the genital tract. In addition, they are potentially safe to use, since they are highly controlled for viruses and other contaminants. Therefore, co-culture using Vero cells has been widely utilized to enhance embryo viability and development, although not without controversies. We thus designed a series of experiments to demonstrate whether Vero cells do indeed enhance mouse embryo development as well as to compare the efficacy of co-culturing mouse 1-cell embryos on Vero cell monolayer in both Ham's F-10 and human tubal fluid (HTF) culture media. 1-cell stage ICR mouse embryos were cultured either in the presence of Vero cells (Group A) or in conventional culture medium alone (Group B). In Ham's F-10 significantly more 3-to-8cell embryos developed in group A than group B (59.8 versus 10.0%; p<0.01). In contrast, there was no significant difference in embryonic development both group A and group B in HTF. However, significant differences were noted only in later embryonic stage (13 and 0%; p<0.05 of group A and B respectively, hatching or hatched). In Ham's F-10, we also could observe the beneficial effect of Vero cell on hatching process (70.7 and 42.1%; p<0.05 of group A and group B respectively).
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