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대한생식의학회지 제24권 제2호 2010년
인간 미성숙난자의 동결.융해후 체외 배양된 난자에 대한 염색체 분석
포천중문의과대학교 차병원 여성의학연구소;포천중문의과대학교 차병원 여성의학연구소;포천중문의과대학교 차병원 여성의학연구소;포천중문의과대학교 차병원 여성의학연구소;포천중문의과대학교 차병원 여성의학연구소;포천중문의과대학교 차병원 여성의학연구소;포천중문의과대학교 차병원 여성의학연구소;포천중문의과대학교 차병원 여성의학연구소;포천중문의과대학교 차병원 여성의학연구소;
박성은;정창조;손원영;정형민;이숙환;이우식;고정재;윤태기;차광열;,
Chromosome Configurations of Human Oocytes Matured in vitro following Cryopreservation at the Germinal Vesicle Stage
Park, S.E.;Chung, C.J.;Son, W.Y.;Chung, H.M.;Lee, S.H.;Lee, W.S.;Ko, J.J.;Yoon, T.K.;Cha, K.Y.;
Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University;Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University;Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University;Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University;Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University;Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University;Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University;Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University;Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University;
Objective: To investigate effects of cryoprotectant and cryopreservation on the chromosome of the human immature oocytes. Design: Intact cumulus-enclosed immature oocytes were collected from unstimulated ovaries and divided into three groups, such as no treatment as control (group 1), only 1,2-propanediol (PROH)-treated (group 2), and cryopreserved oocytes (group 3). Oocytes in group 1, 2, and survived oocytes after cryopreservation in group 3 were cultured for 48 hours. Setting: Infertility Medical Center at the CHA General Hospital, Seoul, Korea. Patients: Oocytes were obtained from Patients undergoing gynecological surgery. Main Outcome Measures: Maturation rate, abnormality in chromosomes by fluorescence in situ hybridization (FISH). Results: There was no effect of PROH only treatment on the chromosomal abnormalities in group 2 compared to control oocytes (41.4% and 31.8%, respectively). Whereas significantly increased abnormalities in chromosome (77.8%) were found in group 3. Conclusions: Human oocytes matured in vitro after cryopreservation at the germinal vesicle (GV) stage showed increased incidence of chromosomal abnormalities. These abnormalities may impair the capacity for further development of the embryos derived from frozen-thawed oocytes.
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