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대한생식의학회지 제25권 제2호 2010년
NO(Nitric Oxide)가 생쥐의 배 발달에 미치는 영향
원광대학교 의과대학 산부인과학 교실, 원광대학 약학대학
민부기, 김기석, 이희섭, 홍기연, 신형도, 성연경, 김형민,
The Effect of Nitric Oxide on the Embryonal Development in Mouse
Min bk, Kim ks, Rhee hs, Hong gy, Shin hd, Sung yk, Kim hm
Objective: To ananlyze the direct effect of nitric oxide(NO), generated from sodium prusside(SNP)on the embryo developments in reproductive process. Design: Ova from mouse were treated to allow fertilization in in vitro culture. And the samples of fertilized ova were alloted into five alliquots. Each alliquot was cultured in media treated with either concentration at 0(n=92), 25mM(n=84), 50mM(n=80), 100mM(n=77), 500mM(n=54) of SNP. Main Outcome Measure: rates of embryonal cell cleavages, viability and cell morphology were assessed during in vitro fertilization and culture. Results: As analyse the cell cleavage at 24 hours after in vitro culture of fertilised egg in variuos NO concentration, all of egg cells of each alliquot were developed to 2-4 cell stage. But the alliquot of egg cells treated with 500mM, which were totally degenerated. And also all embryonal cells of each alliquot were developed to 8 cell stage and morula stage on culture continuosly. And the embryonal cells of each alliquot were analysed at 24 and 48 hours following the in vitro culture, The rates of cell fragmentation and fusion were 4.2¡¾3.4% in control group which is not treated with NO, while experimental groups was high, as rated 23.4¡¾6.2% in 25mM, 28.2¡¾5.7% in 50mM and 32.1¡¾6.4% in 100mM concentration of NO. Accordingly the rate of abnormal morphology of embryonal cells in control was lower significantly than that in each alliquot of experimental group(P<0.05). And the degenerated rates of embryonal cells were 0% in control, 17.8¡¾6.7 in 25mM, 23.6¡¾4.7 in 50mM and 26.8¡¾11.2% in 100mM at 8 cells and morula on culture of 48 and 72 hours. On the examination of embryonal cells developed to blastocyst through in vitro culture, the rates of degenerated cells were 16.8¡¾7.2% in control, 37.5¡¾6.2% in 25mM, 73.4¡¾4.6% in 50mM, 100% in 100mM. Conclusion: This results suggeted that the NO in any concentrations is harmful on embryos in view of morphology as well as viability of cell, and the toxicity of NO on embryo is stronger at condition in higher concentration of NO
키워드 : NO concentration, Embryonal development, Toxicity of NO.
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