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대한생식의학회지 제25권 제2호 2010년
동결-융해 후 회수된 고환 정자와 세정관내 정자의 수정 능력과 효율성에 관한 연구
삼성제일병원 불임연구실1,비뇨기과2, 산부인과3, 성균관대학교 의과대학4
박용석, 전진현, 이호준, 김종현, 서주태, 이유식, 강인수,
Efficacy and Fertilizing ability of Frozen-thawed Testicular Spermatozoa and Spermatozoa Extracted from the Seminiferous Tubule with Intracytoplasmic Sperm Injection (ICSI)
Park ys, Jun jh, Lee hj, Kim jh, Seo jt, Lee ys, Kang is
The combination of testicular sperm extraction (TESE) with ICSI can achieve normal fertilization and pregnancy rate and is effective method in obstructive and non-obstructive azoospermic patients. But, when pregnancy was not occurred, repeated testicular biopsies are not evitable. Therefore in this study, we observed the survival rate of testicular spermatozoa and spermatozoa extracted from the seminiferous tubules after cryopreserved and subsequently used for next IVF cycle with ICSI. In 23 cases, obstructive azoospermia was 17 cases and non-obstructive azoospermia was 6 cases. In obstructive azoospermia, after thawing, motile spermatozoa was observed in 12 cases (70.6%). The fertilization rates with 2PN was 82.6% and 5 pregnancies were achieved 35.3%. In non-obstructive azoospermia, motile spermatozoa after thawing was observed in 2 case (33.3%). The fertilization rates with 2PN was 81.8% and 3 pregnancies (50%) were achieved. A comparison of the results of motile spermatozoa after thawed testicular spermatozoa and spermatozoa extracted from the thawed seminiferous tubule section were 3 cases (60%) and 12 cases (66.6%), respectively. The fertilization and pregnancy rates of thawed testicular spermatozoa and spermatozoa extracted from the thawed seminiferous tubule section were 69.4% and 20%, 62.5% and 38.9%, respectively. Conclusively, thawed testicular spermatozoa and spermatozoa extracted from the thawed seminiferous tubule section can achieve normal fertilization and pregnancy rate and cryopreservation of testicular spermatozoa and seminiferous tubule may avoid repitition of testicular biopsies in azoospermic patients in whom the only source of spermatozoa is the testis.
키워드 : cryopreservation, testicular spermatozoa, seminiferous tubule, TESE, ICSI
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