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대한생식의학회지 제25권 제1호 2010년
항정자항체가 정액성상 및 수정능력에 미치는 영향
서울대학교 의학연구원 인구의학연구소, *서울대학교 의과대학 산부인과학교실
방명걸, 문신용,
The effects of isotypes and regional distribution of antisperm antibodies on semen parameters and fertilizing ability
Pang mg, Moon sy
항정자항체의 종류 및 존재부위가 정액성상 및 수정능력에 미치는 영향을 조사하였다. 항정자항체의 종류 및 존재부위는 immunobead binding test에 의하여 시행하였으며, 정자의 수정능력은 투명대 제거 햄스터 난자 침입법에 의하여 시행하였다. 항정자항체는 정자수, 운동성 및 운동지수에 악영향을 끼쳤으며, 수정능력에도 악영향을 끼쳤다. 항정자항체의 존재부위에 따른 차이는 보이지 않았다. 항정자항체 IgG가 정자두부 혹은 정자미부에 존재할 경우 및 항정자항체 IgA가 정자미부에 존재할 경우 수정능력을 크게 감소시켰다.
It is well-established that antisperm antibodies (ASAs) are detectable in either the male or female partner in a significant proportion (approximately 10%) of infertile couples (Pattinson et al., 1987; Collins et al., 1993). Antibodies to sperm are most often detected in couples with unexplained infertility; they may impair fertility by interfering with sperm cervical mucus penetration, with sperm transport in general, and, in particular, with penetration of the oocyte-surrounding layers, with the integrity of sperm cell membranes, with sperm capacitation and acrosome reaction, with zona pellucida binding and penetration, and with zygote development (Acosta et al., 1994). There are three major types of ASAs in humans: immunoglobulin(Ig)G, IgA, and IgM; each can be directly bound to a patient's spermatozoa, as detected by the immunobead binding test (IBT). IBT has definite advantages over conventional ASA tests because it provides information on the regional distribution of antibodies on the sperm surface and simultaneously allows determination of the proportion of antibody-coated motile spermatozoa (Clarke, 1990). Several studies have attempted to establish a correlation between ASAs and fertilization results in vitro; it is widely agreed that a high antibody titer and binding of antibody to the sperm head are the most powerful predictors of fertilization failure (Zouari et al., 1993; Yeh et al., 1995; Ford et al., 1996), and the prognosis is further worsened by other adverse semen parameters (Acosta et al., 1994; Lahteenmaki, 1993). The presence of surface-bound antibodies on motile spermatozoa, especially the IgA class, has been implicated in IVF impairment (Clarke et al., 1985). However, they have been few reports of the impact of Ig isotypes and the regional distribution of ASA on the human sperm surface on either fertilization results in vitro (Clarke et al., 1985; Witkin et al., 1992) or semen quality (Mathur et al., 1986; De Almeida et al., 1991). To more clearly understand the influences of Ig isotypes and the regional distribution of ASA on the human sperm surface on both fertilizing capacity and semen parameters, we evaluated fertilizing capacity using sperm penetration assay (SPA); semen parameters were evaluated using different classes of antibody present in different regions of spermatozoa.
키워드 : antisperm antibody, antisperm antibody isotype, binding site of antisperm antibody, fertilizing capa
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