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대한생식의학회지 제25권 제1호 2010년
체세포의 공배양체계에서 단일 에너지원이 인간 배반포기 배의 형성에 미치는 영향
한미산부인과의원 불임센터1, 대구대학교 축산학과2
박기상1,2, 최인경1,2, 이진식1, 송해범2,
The Effects of Glutamine on Blastulation of Human Embryos on Vero Cells In Vitro
Park ks, Choi ik, Lee js, Song hb
This study was conducted to investigate the effects of Tissue Culture Medium 199 (TCM) and Dulecco's Modified Eagle Medium (DMEM) on the blastulation and grade of human oocytes on Vero cells in vitro. A cohort of 79 and 93 oocytes in metaphase II stage were used in TCM 199 and DMEM respectively. No differences were found in the numer of oocytes showing two-pronuclei between TCM (82.3%) and DMEM (86.0%). The number of fertilized oocytes reaching the blastocyst was not significant in TCM (60.0%) and DMEM (63.1%). A total of 89 blastocysts were categorized into the four grades (BG1, BG2, BG3 and early) depending on their morphology. The number of embryos achieving the blastocyst grade 1 (BG1) was significantly higher (P<0.05) in DMEM than TCM, at 50.8% (33 out of 65) and 15.0% (12 out of 80) respectively. It is concluded that cultured oocytes in DMEM with glutamine on Vero cells should be significantly increased BG1. key words : Tissue Culture Medium 199 (TCM), Dulbecco's Modified Eagle Medium (DMEM), Two-pronuclei, Blastulation, Blastocyst, Blastocyst grade 1 (BG1)
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