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대한생식의학회지 제30권 제4호 2010년
생쥐 초기 2-세포 배에서 세포 내 칼슘 농도의 변화에 Ni2+이 미치는 영향
성신여자대학교 자연과학대학 생물학과
윤숙영, 이은미,배인하
The effect of Ni2+ on the intracellular Ca2+ increase of the mouse early 2-cell embryos
Yoon SY
Department of Biology, College of Natural Sciences, Sungshin Women's University
Objective: We reported the overcoming effect of Ni2+ on the in vitro 2-cell block of mouse embryos. In this study, we aim to investigate whether Ni2+ should induce intracellular Ca2+ transient in the mouse embryos. Materials and Methods: Embryos were collected at post hCG 32hr from the oviduct of the ICR mouse and cultured in M2 medium omitted phenol red. Intracellular Ca2+ was checked by using a confocal laser scanning microscope and fluo-3AM by using various intracellular Ca2+ antagonists. Results: In 1mM Ni2+ treated medium which contained Ca2+(1.71mM), 75.7% of the embryos showed [Ca2+]i transient about 200 sec later. In the Ca2+-free medium, 69.8% of the embryos showed [Ca2+]i transient. In U73122, phospholipaseC(PLC) inhibitor (5uM, 10min) pretreated group, 33.3% of the embryos showed [Ca2+]i transient. Heparine, inositol 1,4,5-triphosphate receptor(IP3R) antagonist preinjected embryos showed no response with 1mM Ni2+. In danthrolene treatment, ryanodine receptor(RyR)-antagonist , 43% embryos showed [Ca2+]i transient but they showed delayed response about 340sec in the presence of Ca2+. Conclusions: Summing up the above results, Ni2+ seems to induce Ca2+-release from the Ca2+-store even in the Ca2+-free medium. IP3 receptors of the mouse 2-cell embryos might have an essential role for the intracellular Ca2+ increase by Ni2+.
키워드 : mouse in vitro 2-cell block, intracellualr Ca2+ increase, Ni2+, IP3 antagonist, ryanodine receptor a
교신저자 : ihbae@sungshin.ac.kr
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