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대한생식의학회지 제27권 제2호 2010년
자궁내막증 환자의 자궁내막내 성호르몬 수용체, Integrins, Cyclooxygenase의 발현과 변이 양상
아주대학교 의과대학 산부인과학교실1, 분자과학기술과2, 해부병리학교실3, 연세대학교 의과대학 산부인과학교실4
김미란1, 박동욱1, 권혁찬1,2, 황경주1, 주희재3, 조동제4, 김세광4, 오기석1,
Expressiong Pattern of Progesterone Receptor, Integrin, Cyclooxygenase (COX) in Human Endometrium of Patients with Endometrosis
Mi Ran Kim1, Dong Wook Park1, Hyuch Chan Kwon1,2, Kyoung Joo Hwang11, Hee Jae Joo3, Dong Jae Cho4, Sei Kwang Kim4, Kie Suk Oh1
Department of Obstetrics and Gynecology1, Department f Molecular Science and Technology2, Department of Pathology3, Ajou University School of Medicine, Suwon, Department of Obstetrics and Gynecology, Yonsei University College of Medicine4, Seoul, Korea
Objectives: To develop a new immunohistochemical marker system for supplementation of the Noyes histological classification of the endometrium in women of child bearing age with regular menstrual cycles, and to employ this system to evaluate pathologic factors involved in endometriosis, and thus to ascertain if it is useful in diagnosis. Materials and Methods: Endometrial biopsies were sampled from the posterior fundus of 41(24 proliferative phases, 17 secretory phases) women with regular menstrual cycles (28-32 days), and each sample was immunohistochemically stained according to Noyes et al (1975) for determination of expression for estrogen receptor (ER), progesterone receptor (PR), integrin ∝1, ∝4, β3, COX-1 and COX-2. Then, the PR, integrin β3, and COX-2 which were clearly expressed in the luteal phase was with endometrial samples were obtained from 20 cases of normal patients (group 1) and 25 cases with endometriosis (group 2) after confirming the day of ovulation by sex steroid level measurements 7-8 days after ovulation. Results: In the regular menstruation group the expression of ER showed a tendency to be increased in the proliferative phase and decreased in the secretory phase, and was the highest in the proliferative phase. However, PR in the stromal cells showed no change in the entire menstrual cycle while in the epithelial cells, PR reached a peak in the late proliferative phase and was almost absent in the secretory phase. Integrin ∝1, ∝4, and β3 expression in the epithelial cells was absent in the proliferative phase but ∝1 was strongly expressed starting from the early secretory phase into the entire secretory phase. ∝4 was expressed strongly in the early and mid secretory phases and disappeared in the late proliferative phase, while β3, appeared after the mid secretory phase ad continued to be expressed until the late secretory phase. Expression in the stromal cells was weak overall and did not show any cyclic pattern. COX-1 expression was shown as a cyclic pattern in the stromal and epithelial cells and was particularly strongly expressed in the mid secretory phase of epithelial cells, and in the mid secretory and menstruation phase of stromal cells. In the endometrial epithelial cells there was strong expression during the entire cycle with stronger expression in the secretory phase compared to the proliferative phase. COX-2 was clearly expressed in the late proliferative, early and mid secretory phase compared to the proliferative phase. COX-2 was clearly expressed in the late proliferative, early and mid secretory phases in the stromal cells. No expression was observed in the proliferative phase of the epithelial cells, but which began to appear in the early secretory phase reaching a significant pattern form the mid secretory phase onwards. There was almost no expression in the stromal cells. In the cases with endometriosis showing normal endometrial maturation according to the Noyes classification, PR expression was increased while Integrin-β3의 expression was significantly decreased compared to the normal group. Also, COX-2 expression was slightly decreased in the stromal cells of patients with endometriosis while it was significantly increased in the stromal cells. Conclusion: Immunohistochemical markers can supplement the Original Noyes Classification of histological endometrial dating and therefore ascertain existing pathologic conditions. Particularly for patients with endometriosis with normally mature endometrial cells, changes in COX-2 and integrin expression patterns may assist in elucidating pathophysiologic mechanisms and therefore aid in the diagnosis of abnormal implantation conditions, and consequently determine a treatment modality.
키워드 : Implantation, Endometriosis, Sex steroid receptor, Cyclooxygenase, Integrin
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