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대한생식의학회지 제27권 제4호 2010년
인간정자의 처리에 있어서 Percoll과 Sil-Select 방법의 비교
서울대학교 의과대학 산부인과학교실1, 서울대학교 의학연구원 인구의학연구소2, 성균관대학교 의과대학 삼성제일병원 산부인과3
문신용12, 류범용2, 신현아2, 오선경2, 서창석1,2, 김석현1,2, 최영민1,2, 김정구1, 최규홍2,3, 이진용1,
Comparison between Percoll and Sil-Select Methods on the Human Spermatozoa Treatment
Shin Yong Moon1,2, Buom Yong Ryu2, Hyun Ah Shin2, Sun Kyung Oh2, Chang Suk Suh1,2, Seok Hyun Kim1,2, Young Min Choi1,2, Jung Gu Kim1, Kyu Hong Choi2,3, Jin Yong Lee1
Department of Obstetrics and Gynecology, College of Medicine1, Institute of Reproductive Medicine and Population, Medical Research Center2, Seoul National University, Department of Obstetrics and Gynecology, Samsung Cheil Hospital, Sungkyunkwan University, School of medicine3, Seoul, Korea
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Objective: To evaluate silane-coated silica particles (Sil-select) as an alternative to polyvinylpyrrolidone-coated particles (Percoll) for gradient separation of spermatozoa, for use in assisted reproduction. Methods: 20 normal semen based on WHO criteria were included in this study. Recovery of motile and morphologically normal spermatozoa after using two-layer Percoll and Sil-select gradient respectively was recorded. Motility, HOST (hypoosmotic swelling test) and the detection of malondialdehyde for LPO (lipid peroxidation) after 24 h of incubation at 37℃ in a 5% CO2 incubator were compared. Results: Percoll (78.5%) and Sil-select (79.1%) showed a significant increase in the motility compared to ejaculate (60.9%) but no difference between Percoll and Sil-select Normal sperm morphology significantly increased after Percoll (57.6%) and Sil-select (53.7%) compared to ejaculate (35.8%) but no difference between Percoll and Sil-select. No differences in the recovery of motile spermatozoa and motility, HOST and the production of malondialdehyde after 24 h incubation were found when comparing the use of Percoll and Sil-select. Conclusion: Sil-select seems to be an attractive alternative to Percoll for sperm separation in assisted reproduction.
키워드 : Sil-select, Percoll, Gradient separation, Assisted reproduction, Motility
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