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대한생식의학회지 제27권 제4호 2010년
용해된 Matrigel 첨가 배지에서 착상전 생쥐 배아의 발생
1경기대학교 자연과학부 생물학과, 2경기대학교 인문예술총괄학부, 3울산대학교 의과대학 산부인과학교실
정병목1, 추형식2, 강병문3, 계명찬1,
Development of Mouse Preimplantation Embryos in Solubilized Matrigel Media
Byung Mok Chung1, Hyung Sik Choo2, Byung Moon Kang3, Myung chan Gye1
1Department of Biology, College of Natural Sciences, Kyonggi University, 2Faculty of Liberal Arts, Kyonggi University, 3Department of Obstetrics and Gynecology, Collage of Medicine, Ulsan University
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Objective: To verify the effect of two forms (growth factor and growth factor-reduced) of solubilized Matrigel on the development in mouse preimplantation embryos. Methods: Late 2-cell stage eggs were cultured through the blastocyst stage in the presence of GF- or GFR-Matrigel (0.5%, v/v). Morphological development, cell number and % apoptotic nuclei of blastocyst were measured by Hoecst staining and TUNEL of nuclei. Results: Morphological development, number of cells per embryo was significantly increased in the presence of GF- or GFR-Matrigel. Culture of the embryos in the GF-Matrigel gave the best result. Conclusion: Low concentration of solubilized Matrigel improved development of mouse embryos regardless of growth factor content of the Matrigel. Growth factors and extracellular matrix protein included in the Matrigel synergistically potentiated the development of mouse embryos.
키워드 : Matrigel, Mouse embryos, Development
교신저자 : mcgye@kuic.kyonggi.ac.kr
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