| 학회지 검색 | HOME > 논문 및 학회지 > 학회지 검색 |
대한생식의학회지 제27권 제4호 2010년
생쥐 Preantral 난포의 체외배양: FSH의 종류와 농도 및 초자와 냉동보존의 영향1
차병원 여성의학연구소2, 포천중문 의과대학교1
이숙현2, 신창숙2, 정형민2,3, 고정재2,3, 차광렬2,3, 이경아2,3,
In Vitro Culture of the Isolated Mouse Preantral Follicles: Effect of Different Types of FSH and Vitrification1
Sook Hyun Lee2, Chang Sook Shin2, Hyung Min Chung2,3, Jung Jae Ko2,3, Kwang Yul Cha2,3, Kyung-Ah Lee2,3
Infertility Medical Center2, CHA General Hospital, College of Medicine, Pochon CHA University3, Soeul, 135-081, Korea
.
Objective: 1) To compare efficacy of urofollitropin (Follimon) to that of recombinant human FSH (rhFSH) on the growth and maturation of mouse early preantral follicles in vitro, and 2) effect of vitrification on the growth and maturation of preantral follicles and oocytes. Methods: Isolated early preantral follicles (100~130 um diameter) were cultured for 12 days in 20 ul a-MEN media drop under the mineral oil. Follimon or rhFSH was added to the culture medium at various concentrations (0, 10, 100, and 1000 mIU/ml). Results: With Follimon, the dose of 10 mIU/ml showed the best follicle survival, growth, and MII rate of oocyte than the other concentrations. Whereas the optimal Dose of thFSH was 100 mIU/ml. Despite the different optimal doses, the efficacy of two different FSHs on the follicle growth and maturation was similar. Isolated mouse preantral follicles were cryopreserved by vitrification and cultured in vitro for 12 days with 100 mIU/ml rhFSH. Despite the decreased follicular survival rate after thawing, the follicular growth and maturation rate of its oocyte were comparable to those of the fresh follicle. Conclusion: Results from the present study revealed that 1) the optimal doses of Follimon and rhFSH for in-vitro culture of mouse follicles are different, and 2) the frozen-thawed follicles develop normally after vitrification.
키워드 : Follimoon/ Recombinant human FSH/In-vitro follicle culture/ Mouse/ Vitrification
교신저자 : .
전문 파일 : 
