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대한생식의학회지 제16권 제2호 2010년
생쥐 정자의 운동성에 미치는 Calcium 과 dbcAMP 의 영향
한양대학교 자연과학대학 생물학과;한양대학교 자연과학대학 생물학과;한양대학교 자연과학대학 생물학과;한양대학교 자연과학대학 생물학과;충북대학교 자연과학대학 생물학과;
김문규;오승훈;계명찬;윤현수;이준영;,
Effects of Calcium and dbcAMP on Sperm Motility in Mouse
Kim, Moon-K.;Oh, Seung-H.;Gye, Myung-C.;Yoon, Hyun-S.;Lee, Joon-Y.;
Department of Biology, College of Natural Sciences , Hanyang University;Department of Biology, College of Natural Sciences , Hanyang University;Department of Biology, College of Natural Sciences , Hanyang University;Department of Biology, College of Natural Sciences , Hanyang University;Department of Biology, College of Natural Sciences, Chungbuk University;
In order to study the effects of $Ca^{++}$ and dbcAMP on sperm motility, cauda epididyal spermatozoa of mouse were incubated in the media containing the various concentrations of $Ca^{++}$ and dbcAMP, and Its motility were observed and analyzed. The rate(%) of the sperm with forward motility which may be fertile, reached at the equilibrium state in 30-60 min. after the beginning of incubation. $Ca^{++}$ requirement for the maintenance of sperm motility obviously appeared in 3hrs. after the beginning of incubation. In the medium containing 0.01mM $Ca^{++}$, the rate of sperm forward motility was highest(30.6-40.6%), whereas in 10mM $Ca^{++}$ medium, the rate was rather reduced because of a severe agglutination phenomenon. On the other hand, 1mM dbcAMP in the medium was significantly effective for the maintenance of sperm forward motility.
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