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대한생식의학회지 제16권 제2호 2010년

인간 난자의 체외수정을 위한 정도관리로서 생쥐 착상전 배아의 배양에 관한 연구

전북대학교 의과대학 산부인과학교실;전북대학교 의과대학 산부인과학교실;전북대학교 의과대학 산부인과학교실;전북대학교 의과대학 산부인과학교실;

이기숙;박종덕;이춘근;김종덕;,

Mouse Embryo Culture as Quality Control for Human IVF:Culture Media and Supplements

Lee, Gy-Soog;Park, Jong-Duk;Lee, Choon-Khoon;Kim, Jong-Duk;

Department of Obstetrics and Gynecology, College of Medicine, Chonbuk National University;Department of Obstetrics and Gynecology, College of Medicine, Chonbuk National University;Department of Obstetrics and Gynecology, College of Medicine, Chonbuk National University;Department of Obstetrics and Gynecology, College of Medicine, Chonbuk National University;

The development of 2-cell mouse embryos to the blastocyst stage in vitro has been used as quality control for the culture media and supplements employed for human in vitro fertilization and embryo transfer(IVF-ET). 2-cell mouse embryos were cultured to the blastocyst stage in SECM, Medium 199-Earle's, Ham's F-10 I , Ham's F-10 II , Hoppe & Pitts, MEM and $HT_6$. The protein supplements contained in media were bovine serum albumine, fetal bovine serum and human fetal cord serum. The results were as follows; 1. The successful development was 81.3% in Medium 199-Earle’s, 91.9% in Ham’s F-10 I and 97.1% in $HT_6$. 2. 2-cell mouse embryos developed properly in all supplements but the best development was particularly noted in $HT_6$ media when HFCS was supplied as protein supplement.

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