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대한생식의학회지 제19권 제1호 2010년

생쥐배의 생존성 평가에 있어 FDA의 이용

경희대학교 의과대학 산부인과학교실(불임크리닉);경희대학교 의과대학 산부인과학교실(불임크리닉);경희대학교 의과대학 산부인과학교실(불임크리닉);경희대학교 의과대학 산부인과학교실(불임크리닉);건국대학교 축산대학 축산학과;

김재명;홍진기;서병희;이재현;정길생;,

The Use of FDA to Assess the Viability of Preimplantation Mouse Embryo In vitro

Kim, Jae-Myeoung;Hong, Jin-Ki;Suh, Byung-Hee;Lee, Jae-Hyun;Chung, Kil-Sheoung;

Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University;Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University;Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University;Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University;College of Animal Husbandary, Kon Kuk University;

A fluorescence microscopy technique using flurescein diacetateCFDA) as a substract has been tested for the evaluation of the viability of early mouse embryos. Embryos were incubated in T6 containing FDA concentrations of 2.5 to $50{\mu}g/ml$ for 1 to 5min. Embryos were then examined by reflected light fluorescence using a KP 490 and 520 barrier filter in a Nicon Diaphot microscopy. The results were as follow. 1. The rate of fluorescein accumulation increased on the concentration on FDA from $2.5{\times}10^{-6}M$ to $20{\times}10^{-6}M$ 2. The rate at which intracellular fluorescein was lost from embryos was depended on the temperature at which are stored. 3. Embryos with 3 min exposure to FDA have the most intensity of fluorescence. 4. Exposure of 2 cell embryos to FDA ($2.5-5{\mu}g/ml$) for 1 min did not alter their ability to delope normally in vitro.

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