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대한생식의학회지 제22권 제3호 2010년
사람 다수정난자의 체외배양시 Fragmented Embryo와 Non-fragmented Embryo에서의 Methionine 유입량 및 미토콘드리아 분포양상의 비교
차병원 여성의학연구소;차병원 여성의학연구소;차병원 여성의학연구소;차병원 여성의학연구소;차병원 여성의학연구소;차병원 여성의학연구소;차병원 여성의학연구소;
도병록;정미경;장미경;이경아;고정재;윤태기;차광열;,
Mitochondrial Distribution and Methionine Uptake in Fragmented and Non-fragmented Embryos Derived from Multi-pronuclei Zygotes in Human In Vitro Fertilization (IVF) Program
Do, B.R.;Chung, M.K.;Chang, M.K.;Lee, K.A.;Ko, J.J.;Yoon, T.K.;Cha, K.Y.;
Infertility Medical Center, CHA General Hospital;Infertility Medical Center, CHA General Hospital;Infertility Medical Center, CHA General Hospital;Infertility Medical Center, CHA General Hospital;Infertility Medical Center, CHA General Hospital;Infertility Medical Center, CHA General Hospital;Infertility Medical Center, CHA General Hospital;
Despite the frequent incidence of embryo fragmentation in early human embryos, the reason of the embryo fragmentation has not been known yet. This study was conducted to investigate the histological difference(s) between fragmented (FR) and non-fragmented (NFR) human embryos focusing on comparison of mitochondrial distribution and protein synthesis. Multi-pronuclei zygotes (MPZ) such as three or more pronuclei containing in human in vitro fertilization and embryo transfer (IVF-ET) program were used for this study. MPZ were cultured in TCM-199 supplemented with 10% of human fetal cord serum (hFCS) in 5%
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