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대한생식의학회지 제27권 제4호 2010년

인간정자의 처리에 있어서 Percoll과 Sil-Select 방법의 비교

서울대학교 의과대학 산부인과학교실, 서울대학교 의학연구원 인구의학연구소;서울대학교 의학연구원 인구의학연구소;서울대학교 의학연구원 인구의학연구소;서울대학교 의학연구원 인구의학연구소;서울대학교 의과대학 산부인과학교실, 서울대학교 의학연구원 인구의학연구소;서울대학교 의과대학 산부인과학교실 서울대학교 의학연구원 인구의학연구소;서울대학교 의과대학 산부인과학교실, 서울대학교 의학연구원 인구의학연구소;서울대학교 의과대학 산부인과학교실;서울대학교 의학연구원 인구의학연구소, 성균관대학교 의과대학 삼성제일병원 산부인과;서울대학교 의과대학 산부인과학교실;

문신용;류범용;신현아;오선경;서창석;김석현;최영민;김정구;최규홍;이진용;,

Comparison between Percoll and Sil-Select Methods on the Human Spermatozoa Treatment

Moon, Shin-Yong;Ryu, Buom-Yong;Shin, Hyun-Ah;Oh, Sun-Kyung;Suh, Chang-Suk;Kim, Seok-Hyun;Choi, Young-Min;Kim, Jung-Gu;Choi, Kyu-Hong;Lee, Jin-Yong;

Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University;Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University;Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University;Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University;Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Department of Obstetrics and Gynecology, Samsung Cheil Hospital, Sungkyunkwan University, School of Medicine;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University;

Objective: To evaluate silane-coated silica particles (Sil-select) as an alternative to polyvinylpyrrolidone-coated particles (Percoll) for gradient separation of spermatozoa, for use in assisted reproduction. Methods: 20 normal semen based on WHO criteria were included in this study. Recovery of motile and morphologically normal spermatozoa after using two-layer Percoll and Sil-select gradient respectively was recorded. Motility, HOST (hypoosmotic swelling test) and the detection of malondialdehyde for LPO (lipid peroxidation) after 24 h of incubation at $37^{\circ}C$ in a 5% $CO_2$ incubator were compared. Results: Percoll (78.5%) and Sil-select (79.1%) showed a significant increase in the motility compared to ejaculate (60.9%) but no difference between Percoll and Sil-select. Normal sperm morphology significantly increased after Percoll (57.6%) and Sil-select (53.7%) compared to ejaculate (35.8%) but no difference between Percoll and Sil-select. No differences in the recovery of motile spermatozoa and motility, HOST and the production of malondialdehyde after 24 h incubation were found when comparing the use of Percoll and Sil-select. Conclusion: Sil-select seems to be an attractive alternative to Percoll for sperm separation in assisted reproduction.

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