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대한생식의학회지 제27권 제4호 2010년
생쥐배아의 부화와 탈각에 미치는 Pronase의 영향
서울대학교 의과대학 산부인과학교실, 서울대학교 의학연구원 인구의학연구소;서울대학교 의과대학 산부인과학교실, 서울대학교 의학연구원 인구의학연구소;서울대학교 의과대학 산부인과학교실, 서울대학교 의학연구원 인구의학연구소;서울대학교 의과대학 산부인과학교실, 서울대학교 의학연구원 인구의학연구소;서울대학교 의과대학 산부인과학교실, 서울대학교 의학연구원 인구의학연구소;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;서울대학교 의학연구원 인구의학연구소, 성균관대학교 의과대학 삼성제일병원 산부인과;서울대학교 의과대학 산부인과학교실;
문신용;최성미;김희선;류범용;오선경;서창석;김석현;최영민;김정구;최규홍;이진용;,
Effect of Pronase Treatment on Mouse Embryos: Improving Hatching and Hatched Rates
Moon, Shin-Yong;Choi, Sung-Mi;Kim, Hee-Sun;Ryu, Buom-Yong;Oh, Sun-Kyung;Suh, Chang-Suk;Kim, Seok-Hyun;Choi, Young-Min;Kim, Jung-Gu;Choi, Kyu-Hong;Lee, Jin-Yong;
Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University;Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Department of Obstetrics and Gynecology, Samsung Cheil Hospital, Sungkyunkwan University School of Medicine;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University;
Objective: Hatching of the blastocyst from the zona pellucida (ZP) is a key event in mammalian implantation. In vivo, two factors have been identified as possible mediators of hatching: lysis of the ZP by substances elaborated either from the embryo or female reproductive tract and pressure exerted on the zona by expansion of the blastocyst. Two methods of zona manipulation were already in use to enhance the ability of embryos to hatch: mechanical PZD and chemical ZD by acidic Tyrode's solution. But several controversies of each method have been reported. The purpose of this study was to investigate the effect of pronase for mouse embryo hatching. Methods: Mouse embryos were obtained following ovulation induction of
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