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대한생식의학회지 제27권 제4호 2010년

사람 난포액의 처리 방법과 Sample이 생쥐 수정란의 체외 발달율에 미치는 영향

진주 가야자모병원 불임 유전연구소;진주 가야자모병원 불임 유전연구소;진주 가야자모병원 불임 유전연구소;진주 가야자모병원 불임 유전연구소;경상대학교 과학교육학부;진주 가야자모병원 불임 유전연구소;진주 가야자모병원 불임 유전연구소;

전병균;최연희;조은정;송건호;곽대오;문진수;김광철;,

Effect of Treatment and Samples of Human Follicular Fluid on Development In Vitro of Mouse Embryos

Jeon, Byeong-Gyun;Choi, Yeon-Hee;Jo, Eun-Jung;Song, Gun-Ho;Kwak, Dae-Oh;Moon, Jin-Soo;Kim, Kwang-Chul;

Institute of Human Infertility and Cytogenetics, Chinju Kaya Mother-Child's Hospital;Institute of Human Infertility and Cytogenetics, Chinju Kaya Mother-Child's Hospital;Institute of Human Infertility and Cytogenetics, Chinju Kaya Mother-Child's Hospital;Institute of Human Infertility and Cytogenetics, Chinju Kaya Mother-Child's Hospital;Division of Science Education, Gyeongsang National University;Institute of Human Infertility and Cytogenetics, Chinju Kaya Mother-Child's Hospital;Institute of Human Infertility and Cytogenetics, Chinju Kaya Mother-Child's Hospital;

The present studt was performed to investigate the effect of treatment and samples of human follicular fluid (hFF) on the development in vitro of mouse embryos. The two cell stage embryos collected at 40 h post-hCG injection were cultured in the modified human tubal fluid (m-RTF) containing 15% synthetic serum substitute (SSS) or human tubal fluid (hFF) for up to 3 days at $37^{\circ}C$ in 5% $CO_2$ incubator. Also the composition of hormone, total protein and protein pattern of hFF samples were analyzed. The developmental rate of mouse embryos developed to blastocyst were not significant difference in the m-RTF containing 15% hFF filtered with 0.22 or 0.8 ${\mu}m$ syringe filter, however, the embryos cultured in the m-RTF containing inactivated hFF were significantly (p<0.05) developed at the high rate to blastocyst than those containing fresh hFF and SSS. The in vitro developmental rate to blastocyst and hatched blastocyst in the m-RTF containing 15% hFF sample A (90.5 and 85.4%, respectively) and SSS (79.4 and 75.3, respectively) were significantly (p<0.05) increased, compared with hFF sample B (64.2 and 54.1 %, respectively). The hFF sample A tended to be higher concentration of LH, FSR, total protein and the ratio of progesterone/$E_2$ and lower concentration of $E_2$ and progesterone than the hFF sample B, but there were no differences in the protein pattern between the two hFF samples. The results of these study suggest that the addition of hFF to the culture medium enhances the development in vitro to blastocyst and hatched blastocyst, but the in vitro developmental rate of mouse embryos is different between hFF samples.

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