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대한생식의학회지 제27권 제4호 2010년

생쥐 Preantral 난포의 체외배양: FSH의 종류와 농도 및 초자화 냉동보존의 영향

차병원 여성의학연구소;차병원 여성의학연구소;차병원 여성의학연구소, 포천중문 의과대학교;차병원 여성의학연구소, 포천중문 의과대학교;차병원 여성의학연구소, 포천중문 의과대학교;차병원 여성의학연구소, 포천중문 의과대학교;

이숙현;신창숙;정형민;고정재;차광렬;이경아;,

In Vitro Culture of the Isolated Mouse Preantral Follicles: Effect of Different Types of FSH and Vitrification

Lee, Sook-Hyun;Shin, Chang-Sook;Chung, Hyung-Min;Ko, Jung-Jae;Cha, Kwang-Yul;Lee, Kyung-Ah;

Infertility Medical Cente, CHA General Hospital;Infertility Medical Cente, CHA General Hospital;Infertility Medical Cente, CHA General Hospital, College of Medicine, Pochon CHA University;Infertility Medical Cente, CHA General Hospital, College of Medicine, Pochon CHA University;Infertility Medical Cente, CHA General Hospital, College of Medicine, Pochon CHA University;Infertility Medical Cente, CHA General Hospital, College of Medicine, Pochon CHA University;

Objectives: 1) To compare the efficacy of urofollitropin (Follimon) to that of recombinant human FSH (rhFSH) on the growth and maturation of mouse early preantral follicles in vitro, and 2) effect of vitrification on the growth and maturation of preantral follicles and oocytes . Methods: Isolated early preantra1 follicles (100-130 ${\mu}m$ diameter) were cultured for 12 days in 20 ${\mu}l$ ${\alpha}$-MEM media drop under the mineral oil. Follimon or rhFSH was added to the culture medium at various concentrations (0, 10, 100, and 1000 mIU/ml). Results: With Follimon, the dose of 10 mIU/ml showed the best follicle survival, growth, and MIl rate of oocyte than the other concentrations. Whereas the optimal dose of rhFSH was 100 mIU/ml. Despite the different optimal doses, the efficacy of two different FSHs on the follicle growth and maturation was similar. Isolated mouse preantral follicles were cryopreserved by vitrification and cultured in vitro for 12 days with 100 mIU/ml rhFSH. Despite the decreased follicular survival rate after thawing, the follicular growth and maturation rate of its oocyte were comparable to those of the fresh follicle. Conclusion: Results from the present study revealed that 1) the optimal doses of Follimon and rhFSH for in-vitro culture of mouse follicles are different, and 2) the frozen-thawed follicles develop normally after vitrification.

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