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대한생식의학회지 제27권 제4호 2010년
반응성 산소족이 사람 정자의 운동성에 미치는 영향
을지의과대학, 을지병원 의과학연구소;을지의과대학, 을지병원 의과학연구소;을지의과대학, 을지병원 의과학연구소;을지의과대학, 을지병원 의과학연구소;을지대학병원 산부인과;을지의과대학, 을지병원 의과학연구소, 을지의과대학, 을지병원 생리학교실;한양대학교 생명과학과;
강희규;김동훈;한성원;김묘경;권혁찬;이호준;김문규;,
Effects of Reactive Oxygen Species on Motility in Human Spermatozoa
Kang, Hee-Gyoo;Kim, Dong-Hoon;Han, Sung-Won;Kim, Myo-Kyung;Kwon, Hyuck-Chan;Lee, Ho-Joon;Kim, Moon-Kyoo;
Euji Medical Science Institute, Eulji Medical Center;Euji Medical Science Institute, Eulji Medical Center;Euji Medical Science Institute, Eulji Medical Center;Euji Medical Science Institute, Eulji Medical Center;OB/GYN, School of Medicine, Eulji University;Euji Medical Science Institute, Eulji Medical Center, Department of Physiology Eulji Medical Center;Department of Life Science, College of Natural Science, Hanyang University;
Objective: To investigate the effects of ROS on kinematic parameters in human spermatozoa. To verify the changes in above parameters, human spermatozoa were incubated with xanthine-xanthine oxidase (X-XO), $H_2O_2$, sodium nitroprusside (SNP) or lymphocyte. Otherwise, spemlatozoa were incubated under low $O_2 (5%) condition. Methods: CASA was employed to analyze sperm motion parameters. Results: Under $H_2O_2 treatment, all kinematic parameters of spermatozoa were dramatically increased during 30 min, but gradually decreased thereafter. Under the low concentration of $H_2O_2 (125 ${\mu}M$ and 250 ${\mu}M$), the movement velocity (VAP, VCL, VSL) decreased, but forward movement increased. Under the 1mM $H_2O_2, sperm showed reduced kinematic parameters except during first 30 min of incubation. In the cases of X-XO and SNP treatment, the movement velocity increased but the forward movement reduced. After incubation for 3 hr treatment, the kinematic parameters gradually decreased in high concentration of X-XO. However these parameters maintained or increased in low concentration of X-XO. There was no obvious changes in the above parameters in the high concentration of SNP. In the presence of high concentration of lymphocytes, all parameters decreased. Under the 5% $O_2 condition, the parameters of the movement velocity and movement pattern increased, but forward movement decreased. Taken togethers, it suggested that ROS increased the movement velocity but decreased the forward movement and lateral head replacement. $H_2O_2, X-XO, SNP and lymphocyte treatment significantly increased capacitated spermatozoa within I h of incubation. There was no significant difference in capacitation between low- and high $O_2 group. Conclusion: The early onset of capacitation in the presence of ROS suggest that ROS might be a positive regulator of sperm capacitation and hyperactivation. These results demonstrate that low concentration ROS facilitates the movement velocity but high concentration ROS was inhibitory.
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